shscramble sequence as control (OriGene)
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shscramble sequence as control
Shscramble Sequence As Control, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shscramble+sequence+as+control/Scrambled+shRNA+control+in+pGFP-C-shLenti+shRNA+Vector/pmc11336179-343-27-31
Average 96 stars, based on 206 article reviews
Shscramble Sequence As Control, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shscramble+sequence+as+control/Scrambled+shRNA+control+in+pGFP-C-shLenti+shRNA+Vector/pmc11336179-343-27-31
Average 96 stars, based on 206 article reviews
shscramble sequence as control - by Bioz Stars,
2026-09
96/100 stars
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1) Product Images from "Mdm2 requires Sprouty4 to regulate focal adhesion formation and metastasis independent of p53"
Article Title: Mdm2 requires Sprouty4 to regulate focal adhesion formation and metastasis independent of p53
Journal: Nature Communications
doi: 10.1038/s41467-024-51488-2
Figure Legend Snippet: A , B Tumor spheroid invasion in collagen matrix after ( A ) transfection of HT1080 p53KO cells with siRNAs against Mdm2 or ( B ) treatment with MEL23. Representative images above and quantification below of the area invaded and the number of cells invading 24 h after implantation. C , D Tumor spheroid invasion in the collagen-BME matrix after ( C ) transfection of HT1080 p53KO cells with siRNAs against Mdm2 or ( D ) treatment with MEL23. Representative images and quantification of the area invaded 24 h after implantation. Graphs show three pooled independent experimental replicates, the total number of spheroids in each condition is: siCtrl = 10, siMdm2#1 = 15, siMdm2#2 = 12 in panel ( A ); DMSO = 25, MEL23 = 24 in panel ( B ); siCtrl = 12, siMdm2#1 = 14, siMdm2#2 = 15 in panel ( C ), and DMSO = 8, MEL23 = 9 in panel ( D ). E Representative images of cell morphology of HT1080 p53KO cells transfected with siRNAs against Mdm2 or siCtrl in collagen matrix, 6 h after implantation. F Quantification of more circular cells (circularity of 0.75 or higher) in each condition shown in ( E ), n = 3 groups. The graph represents the average fold change of more circular morphology in Mdm2 silenced cells compared to control in three independent experimental replicates. More details about the quantification can be found in the method’s session. G – I HT1080 p53KO cells stably expressing shRNA scramble (shScramble) or a pool of Mdm2 shRNAs (shMdm2) were used to analyze metastatic burden using mouse models. G Protein levels of Mdm2 and MdmX in HT1080 shScramble and shMdm2 stable cell lines. β-actin was used as a loading control. H , I Representative images above and quantification below of metastatic foci in the lungs after implantation of shScramble or shMdm2 cells using ( H ) orthotropic model, n = 4 mice/group or ( I ) tail-vein model, n = 8 mice/group. In all box and whisker plots the boxes extend from 25 to 75 percentiles and whiskers show min and max values, The line in the center of each box represents the median. Graphs shown in panels ( F , H , I ) represent the mean ± SD of independent experimental replicates. More details about the statistical tests used can be found in the Source Data file.
Techniques Used: Transfection, Control, Stable Transfection, Expressing, shRNA, Whisker Assay
Related Articles
Knockdown:Article Title: Sprouty4 is required for Mdm2 regulation of invasion, focal adhesion formation and metastasis in cells lacking p53 Article Snippet: .. For the single knockdown, HT1080 p53KO cells were transduced with lentivirus carrying a pool of shRNAs against Mdm2 (4 different shRNAs purchased from OriGene, cat.#TL311529) or Article Title: Mdm2 requires Sprouty4 to regulate focal adhesion formation and metastasis independent of p53 Article Snippet: .. For the single Mdm2 knockdown, HT1080 p53KO cells were transduced with lentivirus carrying a pool of shRNAs against Mdm2 (4 different shRNAs purchased from OriGene, cat.#TL311529) or Article Title: Mdm2 requires Sprouty4 to regulate focal adhesion formation and metastasis independent of p53 Article Snippet: .. For the double knock-down, cells expressing shMdm2 or shScramble were transduced with lentivirus carrying a pool of shRNAs against Sprouty4 (four different shRNAs purchased from OriGene, cat.#HC108594) or Transduction:Article Title: Sprouty4 is required for Mdm2 regulation of invasion, focal adhesion formation and metastasis in cells lacking p53 Article Snippet: .. For the single knockdown, HT1080 p53KO cells were transduced with lentivirus carrying a pool of shRNAs against Mdm2 (4 different shRNAs purchased from OriGene, cat.#TL311529) or Article Title: Mdm2 requires Sprouty4 to regulate focal adhesion formation and metastasis independent of p53 Article Snippet: .. For the single Mdm2 knockdown, HT1080 p53KO cells were transduced with lentivirus carrying a pool of shRNAs against Mdm2 (4 different shRNAs purchased from OriGene, cat.#TL311529) or Article Title: Mdm2 requires Sprouty4 to regulate focal adhesion formation and metastasis independent of p53 Article Snippet: .. For the double knock-down, cells expressing shMdm2 or shScramble were transduced with lentivirus carrying a pool of shRNAs against Sprouty4 (four different shRNAs purchased from OriGene, cat.#HC108594) or Article Title: Sprouty4 is required for Mdm2 regulation of invasion, focal adhesion formation and metastasis in cells lacking p53 Article Snippet: .. Cells expressing shMdm2 or shScramble were sorted for GFP positive cells and transduced with lentivirus carrying a pool of shRNAs against Sprouty4 (4 different shRNAs purchased from OriGene, cat.#HC108594) or Sequencing:Article Title: Sprouty4 is required for Mdm2 regulation of invasion, focal adhesion formation and metastasis in cells lacking p53 Article Snippet: .. For the single knockdown, HT1080 p53KO cells were transduced with lentivirus carrying a pool of shRNAs against Mdm2 (4 different shRNAs purchased from OriGene, cat.#TL311529) or Article Title: Mdm2 requires Sprouty4 to regulate focal adhesion formation and metastasis independent of p53 Article Snippet: .. For the single Mdm2 knockdown, HT1080 p53KO cells were transduced with lentivirus carrying a pool of shRNAs against Mdm2 (4 different shRNAs purchased from OriGene, cat.#TL311529) or Article Title: Mdm2 requires Sprouty4 to regulate focal adhesion formation and metastasis independent of p53 Article Snippet: .. For the double knock-down, cells expressing shMdm2 or shScramble were transduced with lentivirus carrying a pool of shRNAs against Sprouty4 (four different shRNAs purchased from OriGene, cat.#HC108594) or Article Title: Sprouty4 is required for Mdm2 regulation of invasion, focal adhesion formation and metastasis in cells lacking p53 Article Snippet: .. Cells expressing shMdm2 or shScramble were sorted for GFP positive cells and transduced with lentivirus carrying a pool of shRNAs against Sprouty4 (4 different shRNAs purchased from OriGene, cat.#HC108594) or Control:Article Title: Sprouty4 is required for Mdm2 regulation of invasion, focal adhesion formation and metastasis in cells lacking p53 Article Snippet: .. For the single knockdown, HT1080 p53KO cells were transduced with lentivirus carrying a pool of shRNAs against Mdm2 (4 different shRNAs purchased from OriGene, cat.#TL311529) or Article Title: Mdm2 requires Sprouty4 to regulate focal adhesion formation and metastasis independent of p53 Article Snippet: .. For the single Mdm2 knockdown, HT1080 p53KO cells were transduced with lentivirus carrying a pool of shRNAs against Mdm2 (4 different shRNAs purchased from OriGene, cat.#TL311529) or Article Title: Mdm2 requires Sprouty4 to regulate focal adhesion formation and metastasis independent of p53 Article Snippet: .. For the double knock-down, cells expressing shMdm2 or shScramble were transduced with lentivirus carrying a pool of shRNAs against Sprouty4 (four different shRNAs purchased from OriGene, cat.#HC108594) or Article Title: Sprouty4 is required for Mdm2 regulation of invasion, focal adhesion formation and metastasis in cells lacking p53 Article Snippet: .. Cells expressing shMdm2 or shScramble were sorted for GFP positive cells and transduced with lentivirus carrying a pool of shRNAs against Sprouty4 (4 different shRNAs purchased from OriGene, cat.#HC108594) or Expressing:Article Title: Mdm2 requires Sprouty4 to regulate focal adhesion formation and metastasis independent of p53 Article Snippet: .. For the double knock-down, cells expressing shMdm2 or shScramble were transduced with lentivirus carrying a pool of shRNAs against Sprouty4 (four different shRNAs purchased from OriGene, cat.#HC108594) or Article Title: Sprouty4 is required for Mdm2 regulation of invasion, focal adhesion formation and metastasis in cells lacking p53 Article Snippet: .. Cells expressing shMdm2 or shScramble were sorted for GFP positive cells and transduced with lentivirus carrying a pool of shRNAs against Sprouty4 (4 different shRNAs purchased from OriGene, cat.#HC108594) or |